Department of Genetics, Centre of Biological Sciences, Kerklaan 30, University of Groningen, Groningen, The Netherlands.
Plant Cell Rep. 1985 May;4(3):151-4. doi: 10.1007/BF00571304.
The selection and characterization of 5-methyltryptophan (5-MT) resistant cell lines is described in a dihaploid potato, clone H(2)578(007). The frequency of resistant calli was increased by treatment of a cell suspension with N-ethyl-N-nitroso-urea. Two lines, 5-MT-21 and 5-MT-26, accumulated tyrosine (160 nMol and 1546 nMol/g fresh callus), and the third, 5-MT-27, accumulated both tyrosine (841 nMol/g fresh callus) and phenylalanine (451 nMol/g fresh callus). In the wildtype tyrosine and phenylalanine content was 65 nMol and 42 nMol/g callus, respectively. The tryptophan content of line 5-MT-26 was significantly increased, from 20 nMol/g to 76 nMol/g fresh callus. The total free amino acid content of the three variant cell lines was higher than that of the wildtype. Variant cell lines 5-MT-21, -26 and -27 showed a low degree of resistance to 5-MT, when grown on a selective medium and were cross-resistant to parafluorophenylalanine and 3-fluorotyrosine, analogues of phenylalanine and tyrosine.
本文描述了在二倍体马铃薯克隆 H(2)578(007)中选择和鉴定 5-甲基色氨酸（5-MT）抗性细胞系的过程。通过用 N-乙基-N-亚硝基脲处理细胞悬浮液，增加了抗性愈伤组织的频率。两条系，5-MT-21 和 5-MT-26，积累了酪氨酸（160 nMol 和 1546 nMol/g 新鲜愈伤组织），第三条系，5-MT-27，同时积累了酪氨酸（841 nMol/g 新鲜愈伤组织）和苯丙氨酸（451 nMol/g 新鲜愈伤组织）。在野生型中，酪氨酸和苯丙氨酸的含量分别为 65 nMol 和 42 nMol/g 愈伤组织。系 5-MT-26 的色氨酸含量显著增加，从 20 nMol/g 增加到 76 nMol/g 新鲜愈伤组织。三种变异细胞系的总游离氨基酸含量高于野生型。变异细胞系 5-MT-21、-26 和-27 在选择性培养基上生长时对 5-MT 表现出低度抗性，并且对对氟苯丙氨酸和 3-氟酪氨酸具有交叉抗性，这两种物质是苯丙氨酸和酪氨酸的类似物。
