Nicák A
Physiol Bohemoslov. 1986;35(2):118-26.
The author describes changes in the disintegration of erythrocytes by brilliant cresyl blue in correlation to age, in rats aged 21, 42, 90-105, 340-360 and 690-720 days. The erythrocytes were incubated for 4 hours in an isotonic NaCl solution, in Krebs-Ringer solution and in each of these solutions plus brilliant cresyl blue. Disintegration in plain NaCl solution was found to be the greatest in the case of erythrocytes from 690- to 720-day-old rats. In the same solution plus brilliant cresyl blue, the rate of disintegration was very high in 21-day-old, 42-day-old and 690- to 720-day-old animals; at 90-105 days it was lower and at 340-360 days it was the lowest. Disintegration of erythrocytes in plain Krebs-Ringer solution was the lowest at 21 and 42 days; in the other age groups it was slightly higher. On adding brilliant cresyl blue, the rate of disintegration rose significantly in 21-, 42- and 690- to 720-day-old animals; at 90-105 days and 340-360 days it was no different from disintegration in plain Krebs-Ringer solution. It can be seen from the results that the rate of brilliant cresyl blue-induced erythrocyte disintegration is dependent on the age of the animals from which the erythrocytes are taken.
作者描述了在21日龄、42日龄、90 - 105日龄、340 - 360日龄和690 - 720日龄的大鼠中，煌焦油蓝导致红细胞崩解的变化与年龄的关系。将红细胞分别在等渗氯化钠溶液、克雷布斯 - 林格溶液以及添加了煌焦油蓝的这两种溶液中孵育4小时。发现在单纯氯化钠溶液中，690 - 720日龄大鼠的红细胞崩解程度最大。在添加了煌焦油蓝的相同溶液中，21日龄、42日龄和690 - 720日龄动物的红细胞崩解速率非常高；90 - 105日龄时较低，340 - 360日龄时最低。在单纯克雷布斯 - 林格溶液中，红细胞崩解在21日龄和42日龄时最低；在其他年龄组中略高。添加煌焦油蓝后，21日龄、42日龄和690 - 720日龄动物的红细胞崩解速率显著升高；90 - 105日龄和340 - 360日龄时与单纯克雷布斯 - 林格溶液中的崩解情况无差异。从结果可以看出，煌焦油蓝诱导的红细胞崩解速率取决于所取红细胞的动物年龄。
