Nicák A
Department of Pharmacology, Faculty of Medicine, Safárik University, Kosice.
Physiol Bohemoslov. 1987;36(5):447-51.
The author describes age-related changes in the disintegration of rat red blood cells (RBC) caused by brilliant cresyl blue (BCB) in isotonic saline containing NaCl and phosphates. Blood was taken from rats aged 21, 45, 90-105, 340-360 and 690-720 days. The RBC were incubated four hours in the given solution. The incubation of RBC from different age groups in isotonic saline with phosphates showed no significant differences between the various age groups as far as disintegration of the RBC was concerned. When BCB was added to the solution, however, there was a marked increase in the disintegration of RBC from 21-, 42- and 690- to 720-day-old rats. The differences found in RBC disintegration can be attributed to age-determined qualitative changes in the structure of the RBC membrane--in the first place, changes in cross-linkages between the chains of the structural proteins of the RBC membrane.
作者描述了在含有氯化钠和磷酸盐的等渗盐水中，煌焦油蓝（BCB）引起的大鼠红细胞（RBC）溶解的年龄相关变化。从21日龄、45日龄、90 - 105日龄、340 - 360日龄和690 - 720日龄的大鼠采集血液。将红细胞在给定溶液中孵育4小时。就红细胞溶解而言，不同年龄组的红细胞在含磷酸盐的等渗盐水中孵育，各年龄组之间未显示出显著差异。然而，当向溶液中加入BCB时，21日龄、42日龄以及690至720日龄大鼠的红细胞溶解显著增加。红细胞溶解中发现的差异可归因于红细胞膜结构中由年龄决定的质的变化——首先是红细胞膜结构蛋白链之间交联的变化。
