Tkácová R, Nicák A, Nistiarová A, Tomori Z, Riemerová M
Department of Pathophysiology, Faculty of Medicine, Safárik University, Kosice.
Physiol Bohemoslov. 1989;38(3):283-6.
The disintegration of erythrocytes by brilliant cresyl blue was studied in rabbits and cats during two hypoxic periods resulting in apnoea which was followed by artificial ventilation and recovery. The rate of erythrocyte disintegration was measured after 2 hours' incubation in isotonic NaCl or Krebs-Ringer solution plus brilliant cresyl blue (0.5 mmol.l-1). Significant increases of disintegration rates were found in rabbits during recovery in both incubation solutions. Erythrocytes in cats seemed to be more resistant to acute hypoxia, as their disintegration rate rose only in isotonic NaCl solution, and that only transiently. Brilliant cresyl blue--induced erythrocyte disintegration in cats did not differ from the control values during 10 min of spontaneous breathing after the first and the second period of hypoxia in the isotonic NaCl solution, but it was significantly lower in Krebs-Ringer solution. The possible factors influencing the erythrocyte disintegration rate in acute hypoxia are discussed.
在兔子和猫身上研究了在两个导致呼吸暂停的缺氧期内，用煌焦油蓝使红细胞溶解的情况，随后进行人工通气并恢复。在等渗氯化钠或 Krebs-Ringer 溶液加煌焦油蓝（0.5 mmol·l⁻¹）中孵育 2 小时后，测量红细胞溶解率。在两种孵育溶液中，兔子恢复期间的溶解率均显著增加。猫的红细胞似乎对急性缺氧更具抵抗力，因为它们的溶解率仅在等渗氯化钠溶液中升高，且只是短暂升高。在等渗氯化钠溶液中，第一次和第二次缺氧期后，猫在 10 分钟自主呼吸期间，煌焦油蓝诱导的红细胞溶解与对照值无差异，但在 Krebs-Ringer 溶液中显著降低。讨论了影响急性缺氧时红细胞溶解率的可能因素。
