油菜叶片原生质体植株再生
Regeneration of plantlets from mesophyll protoplasts of Brassica juncea (L.) Czern.
机构信息
Department of Botany, Bose Institute, 700 009, Calcutta, India.
出版信息
Plant Cell Rep. 1985 Oct;4(5):245-7. doi: 10.1007/BF00269368.
PMID:24253979
Abstract
Isolated mesophyll protoplasts of Brassica juncea (L.) Czern., cv. RLM 514 upon culture in suitable growth medium, regenerated cell wall, underwent cell division and formed cellular colonies. Subsequent induction of embryoid (embryogenesis) and shoot bud (organogenesis) formations in such cell masses resulted in regeneration of 186 and 42 plantlets respectively.
摘要
在适宜的生长培养基中培养的芥菜（ Brassica juncea （L.） Czern.，cv. RLM 514）的分离叶肉原生质体，再生细胞壁，进行细胞分裂并形成细胞集落。随后在这些细胞团中诱导胚状体（胚胎发生）和芽（器官发生）的形成，分别导致 186 和 42 个植株的再生。
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