Department of Genetics, North Carolina State University, 27695-7614, Raleigh, NC, USA.
Theor Appl Genet. 1985 Mar;69(5-6):535-41. doi: 10.1007/BF00251100.
Mitochondrial DNAs from Nicotiana tabacum, an amphiploid, and its putative progenitor species, N. sylvestris and N. tomentosiformis were compared in structure and organization. By using DNA transfer techniques and cloned fragments of known genes from maize and N. sylvestris as labeled probes, the positions of homologous sequences in restriction digests of the Nicotiana species were analyzed. Results indicate that the mitochondrial DNA of N. tabacum was inherited from N. sylvestris. Conservation in organization and sequence homology between mtDNAs of N. tabacum and the maternal progenitor, N. sylvestris, provide evidence that the mitochondrial genome in these species is evolutionarily stable. Approximately one-third of the probed restriction fragments of N. tomentosiformis mtDNA showed conservation of position with the other two species. Pattern variations indicate that extensive rearrangement of mtDNA has occurred in the evolution of these Nicotiana species.
对来自烟草（一种异源多倍体）及其假定祖先种——普通烟草和黄花烟草的线粒体 DNA 的结构和组织进行了比较。利用 DNA 转移技术和克隆的玉米及普通烟草的已知基因片段作为标记探针，分析了限制酶切消化产物中烟草种的同源序列的位置。结果表明，烟草的线粒体 DNA 是从普通烟草中遗传下来的。烟草和母系祖先普通烟草的 mtDNA 在组织和序列同源性方面的保守性，为这些物种的线粒体基因组在进化上是稳定的提供了证据。大约三分之一的黄花烟草 mtDNA 探针的限制片段与其他两个物种在位置上保持一致。图谱变化表明，在这些烟草种的进化过程中发生了广泛的 mtDNA 重排。
