Dale R M, Duesing J H, Keene D
Nucleic Acids Res. 1981 Sep 25;9(18):4583-93. doi: 10.1093/nar/9.18.4583.
Supercoiled mitochondrial DNA (mtDNA) has been isolated in preparative amounts from five different plant species and compared on agarose gels. The gels reveal considerable mtDNA size heterogeneity within each species as well as substantial differences in the range and frequency of the mtDNA size classes among the different plants. The two lowest size classes of three of the plants have been extracted and their molecular weights determined. Comparison of two N. tabacum W38 culture lines has revealed significant differences in their mtDNA size classes. However, despite the totally different supercoil patterns, these two lines have almost identical restriction digests. The availability of preparative amounts of separated mtDNA size classes will make it possible to carry out a detailed analysis of plant mitochondrial genomes.
已从五种不同植物物种中制备性地分离出超螺旋线粒体DNA（mtDNA），并在琼脂糖凝胶上进行了比较。凝胶显示出每个物种内mtDNA大小存在相当大的异质性，以及不同植物之间mtDNA大小类别的范围和频率存在显著差异。已提取了其中三种植物的两个最低大小类别的mtDNA，并测定了它们的分子量。对两个烟草W38培养系的比较显示，它们的mtDNA大小类别存在显著差异。然而，尽管超螺旋模式完全不同，但这两个系的限制性消化图谱几乎相同。可获得制备量的分离mtDNA大小类别将使详细分析植物线粒体基因组成为可能。
