Institute of Physical and Chemical Research, 351, Wako shi, Saitama, Japan.
Planta. 1984 Sep;162(2):154-8. doi: 10.1007/BF00410212.
The soluble fraction of a cell-free system from immature seeds of Phaseolus vulgaris L. converts gibberellin A20 (GA20) to GA1 and GA5. It does however not metabolize GA1 and GA29 to GA5, showing that in this system GA20 is converted directly to GA5. The steps from GA20 to GA1 (3-hydroxylation) and from GA20 to GA5 (Δ(2) double-bond formation) require oxygen, Fe(2+) and α-ketoglutarate, and are stimulated by ascorbate. The enzymes catalyzing these conversions bate. The enzymes catalyzing these conversions have properties similar to those of GA oxidases found in Cucurbita maxima and Pisum sativum.
菜豆未成熟种子的无细胞体系的可溶部分将赤霉素 A20（GA20）转化为 GA1 和 GA5。然而，它不能将 GA1 和 GA29 代谢为 GA5，表明在该体系中 GA20 直接转化为 GA5。从 GA20 到 GA1（3-羟基化）和从 GA20 到 GA5（Δ（2）双键形成）的步骤需要氧气、Fe（2+）和α-酮戊二酸，并被抗坏血酸刺激。催化这些转化的酶。催化这些转化的酶具有与在南瓜和豌豆中发现的 GA 氧化酶相似的性质。
