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在来源于 Phaseolus coccineus L. 的未成熟种子的无细胞体系中，[(14)C]赤霉素 A12-醛向 C19-和 C20-赤霉素的转化

Conversion of [(14)C]gibberellin A 12-aldehyde to C 19- and C 20-gibberellins in a cell-free system from immature seed of Phaseolus coccineus L.

机构信息

Department of Botany, The University, G12 8QQ, Glasgow, UK.

出版信息

Planta. 1985 Jul;165(1):108-13. doi: 10.1007/BF00392218.

DOI:10.1007/BF00392218
PMID:24240964
Abstract

A cell-free system prepared from developing seed of runner bean (Phaseolus coccineus L.) converted [(14)C]gibberellin A12-aldehyde to several products. Thirteen of these were identified by capillary gas chromatography-mass spectrometry as gibberellin A1 (GA1), GA4, GA5, GA6, GA15, GA17, GA19, GA20, GA24, GA37, GA38, GA44 and GA53-aldehyde, all giving mass spectra with (14)C-isotope peaks. GA8 and GA28 were also identified but contained no (14)C. All the [(14)C]GA12-aldehyde metabolites, except GA15, GA24 and GA53-aldehyde, are known endogenous GAs of P. coccineus.

摘要

从菜豆（Phaseolus coccineus L.）发育种子中制备的无细胞体系将[(14)C]赤霉素 A12-醛转化为几种产物。通过毛细管气相色谱-质谱联用技术鉴定了其中的 13 种产物为赤霉素 A1（GA1）、GA4、GA5、GA6、GA15、GA17、GA19、GA20、GA24、GA37、GA38、GA44 和 GA53-醛，它们的质谱均显示出（14)C 同位素峰。GA8 和 GA28 也被鉴定出来，但不含（14)C。除了 GA15、GA24 和 GA53-醛之外，[(14)C]GA12-醛代谢物都是 P. coccineus 的已知内源性 GAs。

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引用本文的文献

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2
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Planta. 1989 Sep;179(2):211-21. doi: 10.1007/BF00393691.
3
Gibberellin biosynthesis from gibberellin A12-aldehyde in a cell-free system from germinating barley (Hordeum vulgare L., cv. Himalaya) embryos.

本文引用的文献

1
The conversion of mevalonic acid into gibberellin A12-aldehyde in a cell-free system from Cucurbita pepo.南瓜细胞无细胞体系中从甲羟戊酸到赤霉素 A12-醛的转化。
Planta. 1972 Sep;102(3):261-71. doi: 10.1007/BF00386896.
2
The biosynthesis of a C19-gibberellin from mevalonic acid in a cell-free system from a higher plant.高等植物细胞无细胞体系中从甲羟戊酸生物合成 C19-赤霉素。
Planta. 1974 Jan;120(3):307-9. doi: 10.1007/BF00390299.
3
Internode length in Pisum : The Le gene controls the 3β-hydroxylation of gibberellin A20 to gibberellin A 1.
在萌发的大麦（Hordeum vulgare L.，cv. Himalaya）胚的无细胞体系中，从赤霉素 A12-醛合成赤霉素。
Planta. 1992 Sep;188(2):252-7. doi: 10.1007/BF00216821.
豌豆节间长度：Le 基因控制赤霉素 A20 的 3β-羟化作用生成赤霉素 A1。
Planta. 1984 Apr;160(5):455-63. doi: 10.1007/BF00429763.
4
The localization, metabolism and biological activity of gibberellins in maturing and germinating seeds of Pisum sativum cv. Progress No. 9.豌豆 cv.Progress No.9 成熟和萌发种子中赤霉素的定位、代谢和生物活性。
Planta. 1983 Nov;159(5):454-68. doi: 10.1007/BF00392082.
5
Conversion of gibberellin A20 to gibberellins A 1 and A 5 in a cell-free system from Phaseolus vulgaris.菜豆细胞无细胞体系中赤霉素 A20 向赤霉素 A1 和 A5 的转化。
Planta. 1984 Sep;162(2):154-8. doi: 10.1007/BF00410212.
6
Identification and localization of gibberellins in maturing seeds of the cucurbit Sechium edule, and a comparison between this cucurbit and the legume Phaseolus coccineus.鉴定和定位葫芦科苦瓜种子成熟过程中的赤霉素，并将这种苦瓜与豆科植物菜豆进行比较。
Planta. 1984 Dec;162(6):560-5. doi: 10.1007/BF00399923.
7
Gibberellins in immature seeds and dark-grown shoots of Pisum sativum : Gibberellins identified in the tall cultivar Alaska in comparison with those in the dwarf Progress No. 9.豌豆未成熟种子和暗培养苗中的赤霉素：高秆品种阿拉斯加与矮秆 9 号品种中赤霉素的比较鉴定。
Planta. 1985 Feb;163(2):283-9. doi: 10.1007/BF00393519.
8
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