使用反射傅里叶变换红外光谱法研究植物细胞壁的酶解。

Investigation of the enzymatic digestion of plant cell walls using reflectance Fourier Transform Infrared spectroscopy.

机构信息

Chemical Laboratories, Department of Life Sciences, University of London Goldsmiths College, New Cross, SE14 6NW, London, UK.

出版信息

Plant Cell Rep. 1985 Dec;4(6):321-4. doi: 10.1007/BF00269889.

DOI:10.1007/BF00269889

PMID:24254073

Abstract

A method has been developed to determine the reflectance Fourier Transform Infrared spectra of plant cells grown in vitro and of the protoplasts released from such cells by enzymatic digestion. It is demonstrated that there is a smooth and reproducible transition in spectral detail as enzymatic digestion procedes. Reflectance Fourier Transform Infrared spectroscopy has been used to monitor the progress of protoplast release during enzymatic digestion of cell wall material.

摘要

已经开发出一种方法来确定在体外生长的植物细胞的反射率傅里叶变换红外光谱，以及通过酶消化从这些细胞中释放的原生质体的反射率傅里叶变换红外光谱。结果表明，随着酶消化的进行，光谱细节呈现出平滑且可重复的转变。反射率傅里叶变换红外光谱已被用于监测细胞壁物质的酶消化过程中原生质体释放的进展。