Mellado Miguel, Garcia Angel M, Arellano-Reynoso Beatriz, Diaz-Aparicio Efren, Garcia Jose E
Department of Animal Nutrition, Antonio Narro Autonomous Agrarian University, Saltillo, Coahuila, 25315, Mexico.
Trop Anim Health Prod. 2014 Feb;46(2):391-7. doi: 10.1007/s11250-013-0502-4. Epub 2013 Nov 20.
The aim of this research was to study if seropositivity for brucellosis in vaccinated cows against this disease hampers reproductive performance and milk production in high-yielding Holstein cows. For this purpose 1,026 healthy cows and 372 cows seropositive for brucellosis were enrolled in this study. Cows positive to card test and subsequently to the rivanol test were further subjected to the radial immunodiffusion (RID) test. It was found that only 11% of the presumably infected cows by brucellosis screening tests were really infected with this disease. The reproductive performance of the group of cows with 11% Brucella-infected animals was not impaired; overall pregnancy rate did not differ between seropositive and healthy cows (30.9 vs. 29.6%). The abortion rates were similar between seropositive cows (5.3%) and seronegative animals (6.9%). Cows in the herd with 11% Brucella-infected animals produced significantly more milk than unaffected cows over a 305-day lactation (10,684 ± 1,720 vs. 10,345 ± 1,736; mean ± SD; P < 0.05). It was concluded that in dairy herds vaccinated against brucellosis with both 19 and RB51 strains, supplemental tests such as RID need to be conducted on all reactors in order to maintain diagnostic accuracy. These results also indicate that 11% animal prevalence of brucellosis did not exert a detrimental effect on 305-day milk yield and reproductive performance in high milk-yielding Holstein cows.
本研究的目的是探讨接种布鲁氏菌病疫苗的奶牛出现血清学阳性是否会影响高产荷斯坦奶牛的繁殖性能和产奶量。为此，本研究纳入了1026头健康奶牛和372头布鲁氏菌病血清学阳性奶牛。对平板凝集试验呈阳性且随后对鲁米那试验呈阳性的奶牛进一步进行放射免疫扩散（RID）试验。结果发现，通过布鲁氏菌病筛查试验推测感染的奶牛中，只有11%确实感染了该病。布鲁氏菌感染动物占11%的奶牛组的繁殖性能未受损害；血清学阳性奶牛和健康奶牛的总体妊娠率无差异（30.9%对29.6%）。血清学阳性奶牛（5.3%）和血清学阴性奶牛（6.9%）的流产率相似。在305天的泌乳期内，布鲁氏菌感染动物占11%的牛群中的奶牛产奶量显著高于未受影响的奶牛（10,684±1,720对10,345±1,736；均值±标准差；P<0.05）。研究得出结论，在使用19号菌株和RB51菌株接种布鲁氏菌病疫苗的奶牛场，需要对所有反应动物进行RID等补充检测，以保持诊断准确性。这些结果还表明，布鲁氏菌病11%的动物感染率对高产荷斯坦奶牛的305天产奶量和繁殖性能没有不利影响。
