Arellano-Reynoso Beatriz, Suárez-Güemes Francisco, Estrada Félix Mejía, Michel-GómezFlores Fernando, Hernández-Castro Rigoberto, Acosta Rómulo Beltrán, Díaz-Aparicio Efrén
Departamento de Microbiología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Circuito Exterior de Ciudad Universitaria, Delegación Coyoacán, Mexico City, C.P. 04510, Mexico.
Trop Anim Health Prod. 2013 Feb;45(2):695-7. doi: 10.1007/s11250-012-0252-8. Epub 2012 Sep 7.
A study was carried out in Pichucalco, Chiapas (Mexico) to determine whether recently calved cows or those that aborted shed Brucella. Serological diagnosis of brucellosis was made in all animals (209). Six of the cows that calved normally and two that aborted underwent a bacteriological study of milk and vaginal exudate. Brucella abortus was isolated from vaginal exudate samples in two 3- to 4-year-old seronegative first-birth cows that had calved normally. This was confirmed through bacteriological identification and PCR as a field strain and smooth phenotypes. We conclude that seronegative cows vaccinated with RB51 which calved normally and shed B. abortus in the vaginal exudate after calving could be a serious problem because these cows are overlooked in routine diagnoses and are a source of Brucella infection.
在墨西哥恰帕斯州的皮丘卡尔科进行了一项研究，以确定近期产犊的母牛或流产的母牛是否会排出布鲁氏菌。对所有动物（209头）进行了布鲁氏菌病的血清学诊断。对6头正常产犊的母牛和2头流产的母牛的乳汁和阴道分泌物进行了细菌学研究。从2头3至4岁、血清学阴性、头胎且正常产犊的母牛的阴道分泌物样本中分离出了牛种布鲁氏菌。通过细菌学鉴定和聚合酶链反应确认这是一株田间菌株且具有光滑型表型。我们得出结论，接种RB51疫苗的血清学阴性母牛正常产犊后在阴道分泌物中排出牛种布鲁氏菌可能是一个严重问题，因为这些母牛在常规诊断中被忽视，并且是布鲁氏菌感染的一个来源。
