Mizutani Plant Ecochemicals Project, Research Development Corporation of Japan (JRDC), 061-13, Eniwa, Japan.
J Chem Ecol. 1992 Oct;18(10):1833-40. doi: 10.1007/BF02751107.
The root exudates fromPolygonum sachalinense in a recirculating system significantly inhibited lettuce seedling growth. The rhizomes and roots ofP. sachalinense were extracted with 80% acetone. Bioassay of the neutral-acidic fraction on the TLC agar plate showed the inhibitory activity corresponded to the two yellow pigment bands. Two orange needles were isolated and identified as anthraquinone compounds: emodin and physcion. Both compounds exhibited inhibitory activities against the seedling growth of several testing plant species. Glucosides were isolated fromP. sachalinense and were identified as emodin-1-O-β-D-glucoside and physcion-1-O-β-D-glucoside, respectively. On plant growth bioassay, these glucosides showed no phytotoxic activity against lettuce seedlings. The concentrations of emodin, physcion, and their glucosides from rhizome with roots, aerial parts, fallen leaves, and soil were determined. The rhizome with roots and fallen leaves contained emodin and physcion at relatively high concentrations. Emodin also occurs in the soil of this plant community with effective concentrations in the fall. The results indicate that these anthraquinones are responsible for the observed interference and are potent allelopathic substances.
在循环系统中，朝鲜蓼的根分泌物显著抑制了生菜幼苗的生长。用 80%丙酮从朝鲜蓼的根茎和根中提取。在 TLC 琼脂板上对中性-酸性部分进行生物测定，显示抑制活性与两条黄色色素带相对应。分离并鉴定出两种橙色针状物为蒽醌化合物：大黄素和大黄素甲醚。这两种化合物均表现出对几种测试植物物种幼苗生长的抑制活性。从朝鲜蓼中分离出葡萄糖苷，并分别鉴定为大黄素-1-O-β-D-葡萄糖苷和大黄素甲醚-1-O-β-D-葡萄糖苷。在植物生长生物测定中，这些糖苷对生菜幼苗没有表现出植物毒性活性。测定了带根、地上部分、落叶和土壤的根茎中大黄素、大黄素甲醚及其糖苷的浓度。带根和落叶的根茎中含有相对较高浓度的大黄素和大黄素甲醚。大黄素也存在于该植物群落的土壤中，秋季的有效浓度较高。结果表明，这些蒽醌类化合物是造成观察到的干扰的原因，是有效的化感物质。
