Institute of Himalayan Bioresource Technology, Palampur, HP, India.
J Plant Physiol. 2010 Jun 15;167(9):749-56. doi: 10.1016/j.jplph.2009.12.007. Epub 2010 Feb 7.
This paper presents quantification of five anthraquinone derivatives (emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion) in rhizomes of hardened micro-propagated Rheum emodi plants using high-performance liquid chromatography (HPLC). Aseptic shoot cultures were raised using rhizome buds. Shoot multiplication occurred in both agar gelled and liquid Murashige and Skoog (MS) medium supplemented with 10.0 microM 6-benzylaminopurine (BAP) and 5.0 microM indole-3-butyric acid (IBA). Rooted plantlets obtained on plant growth regulator (PGR)-free medium were transferred to soil with 92% survival. HPLC analysis revealed the presence of five anthraquinone derivatives: emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion in rhizomes of tissue culture-raised plants. Only emodin glycoside (1) and chrysophanol glycoside (2) were present in 6-month-old hardened tissue cultured plants. In addition, the other three derivatives (emodin (3), chrysophanol (4) and physcion (5)) were also detected after 9 months.
本文采用高效液相色谱法（HPLC）对硬化微繁殖大黄根茎中的五种蒽醌衍生物（大黄苷、大黄酸苷、大黄素、大黄酸和大黄素甲醚）进行了定量分析。无菌芽培养采用根茎芽进行。在琼脂凝胶和液体 Murashige 和 Skoog（MS）培养基中，添加 10.0 μM 6-苄基氨基嘌呤（BAP）和 5.0 μM 吲哚-3-丁酸（IBA），均能发生芽增殖。在无植物生长调节剂（PGR）的培养基上获得的生根苗在 92%的存活率下转移到土壤中。HPLC 分析显示，组织培养植物的根茎中存在五种蒽醌衍生物：大黄苷、大黄酸苷、大黄素、大黄酸和大黄素甲醚。只有 6 个月大的硬化组织培养植物中存在大黄苷（1）和大黄酸苷（2）。此外，在 9 个月后还检测到另外三种衍生物（大黄素（3）、大黄酸（4）和大黄素甲醚（5））。
