Chen Yuan, Zheng Xiaoying, Yan Jie, Qiao Jie, Liu Ping
Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Peking University Third Hospital, Beijing 100191, People's Republic of China.
Reprod Biol Endocrinol. 2013 Nov 21;11:107. doi: 10.1186/1477-7827-11-107.
Increasing evidence indicates that closed vitrification has been successfully used in the cryopreservation of human oocytes and embryos. Little information is available regarding the neonatal outcome of closed blastocysts vitrification. The aim of this study was to evaluate the effectiveness and safety of blastocyst vitrification using a high-security closed vitrification system compared with an open vitrification system.
A total of 332 vitrified-warmed blastocyst transfer cycles between April 2010 and May 2012 were analyzed retrospectively. The post-thaw survival rate, implantation rate, clinical pregnancy rate, live birth rate, and neonatal outcome were recorded.
There were no significant differences between the open vitrification group and the close vitrification group regarding the post-thaw survival rate (98% versus 95.8%), clinical pregnancy rate (47.6% versus 42.2%), implantation rate (42.9% versus 35.6%), and live birth rate (39.8% versus 32.1%). In total, 332 warming cycles produced 131 healthy babies. There were no significant differences in the mean gestational age, the birth weight, and the birth length between the two groups. No adverse neonatal outcomes were observed in the children born after the transfer of closed vitrified blastocysts compared with the transfer of open vitrified blastocysts.
These data suggest that blastocyst vitrification using a closed vitrification device seems safe and effective with results comparable to those obtained through open vitrification.
越来越多的证据表明，封闭式玻璃化冷冻技术已成功应用于人类卵母细胞和胚胎的冷冻保存。关于封闭式囊胚玻璃化冷冻后的新生儿结局的信息较少。本研究的目的是评估与开放式玻璃化冷冻系统相比，使用高安全性封闭式玻璃化冷冻系统进行囊胚玻璃化冷冻的有效性和安全性。
回顾性分析2010年4月至2012年5月期间共332个玻璃化冷冻-解冻囊胚移植周期。记录解冻后存活率、着床率、临床妊娠率、活产率和新生儿结局。
开放式玻璃化冷冻组和解封闭式玻璃化冷冻组在解冻后存活率（98%对95.8%）、临床妊娠率（47.6%对42.2%）、着床率（42.9%对35.6%）和活产率（39.8%对32.1%）方面无显著差异。总共332个解冻周期产生了131名健康婴儿。两组之间的平均孕周、出生体重和出生身长无显著差异。与开放式玻璃化冷冻囊胚移植后出生的儿童相比，封闭式玻璃化冷冻囊胚移植后出生的儿童未观察到不良新生儿结局。
这些数据表明，使用封闭式玻璃化冷冻装置进行囊胚玻璃化冷冻似乎是安全有效的，其结果与开放式玻璃化冷冻相当。
