Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, School of Materials Science and Engineering, Shandong University, Jinan 250061, People's Republic of China.
Analyst. 2014 Jan 21;139(2):488-94. doi: 10.1039/c3an01670f.
Nanoporous gold (NPG) based biosensors have been constructed by covalently immobilizing glucose oxidase (GOx) onto self-assembled monolayers (SAMs). With p-benzoquinone (BQ) as a mediator, diffusion behavior and amperometric biosensor performance are evaluated by electrochemical characterization. The enzyme modified electrodes are demonstrated to show a thickness-sensitive behavior. Compared with planar polycrystalline gold, the unique porous structure of NPG has also been characterized via an electrochemical surface reconstruction process. Single-crystal gold-like electrochemical behavior on NPG and a comprehensive understanding of its impacts on sensor performance have been proposed.
基于纳米多孔金 (NPG) 的生物传感器已通过将葡萄糖氧化酶 (GOx) 共价固定在自组装单层 (SAM) 上来构建。通过电化学特性评估，使用对苯醌 (BQ) 作为媒介，来评估扩散行为和电流式生物传感器性能。结果表明，酶修饰电极具有厚度敏感性。与平面多晶金相比，NPG 的独特多孔结构也通过电化学表面重构过程进行了表征。提出了在 NPG 上类似于单晶金的电化学行为及其对传感器性能的综合影响。
