Department of Dermatology, University of California, Davis, California, USA.
J Virol. 2014 Feb;88(3):1843-8. doi: 10.1128/JVI.03251-13. Epub 2013 Nov 20.
Latent Kaposi's sarcoma-associated herpesvirus (KSHV) episomes are coated with viral latency-associated nuclear antigen (LANA). In contrast, LANA rapidly disassociates from episomes during reactivation. Lytic KSHV expresses polyadenylated nuclear RNA (PAN RNA), a long noncoding RNA (lncRNA). We report that PAN RNA promotes LANA-episome disassociation through an interaction with LANA which facilitates LANA sequestration away from KSHV episomes during reactivation. These findings suggest that KSHV may have evolved an RNA aptamer to regulate latent protein function.
潜伏性卡波氏肉瘤相关疱疹病毒 (KSHV) episomes 被病毒潜伏相关核抗原 (LANA) 包被。相比之下，LANA 在再激活过程中会迅速从 episomes 上解离。裂解性 KSHV 表达聚腺苷酸化核 RNA (PAN RNA)，一种长非编码 RNA (lncRNA)。我们报告称，PAN RNA 通过与 LANA 的相互作用促进 LANA-episome 解离，从而在再激活过程中将 LANA 隔离远离 KSHV episomes。这些发现表明，KSHV 可能已经进化出一种 RNA 适体来调节潜伏蛋白的功能。
