Plant Morphogenesis and Tissue Culture Section, Bio-Organic Division, Bhabha Atomic Research Centre, 400 085, Bombay, India.
Plant Cell Rep. 1982 Oct;1(5):215-8. doi: 10.1007/BF00270239.
Callus cultures were initiated from isolated mature embryos of Triticum turgidum L. Thell ssps durum and dicoccum on a basal medium supplemented with 2,4-D, 2,4,5-Cl3POP or 2,4-D+CM. Shoot bud regeneration was observed on 2,4,5-Cl3POP medium. In both the cultivars of durum, further development of shoot buds occurred on transfer of tissues to basal medium whereas in dicoccum basal medium supplemented with coconut milk or coconut milk with NAA (0.2 mg/l) was necessary. The regenerated shoot buds were induced to root on basal medium supplemented with NAA. The in vitro obtained plants were transferred to soil and successfully grown to maturity. Chlorophyll variants were observed among the regenerated plants of dicoccum.
愈伤组织培养从孤立的成熟胚胎开始，这些胚胎取自硬质小麦（Triticum turgidum L.）的 Thell 亚种 durum 和 dicoccum，在添加了 2,4-D、2,4,5-Cl3POP 或 2,4-D+CM 的基础培养基上。在 2,4,5-Cl3POP 培养基上观察到芽的再生。在 durum 的两个品种中，进一步的芽发育发生在将组织转移到基础培养基上，而在 dicoccum 中，需要在添加椰子奶或椰子奶与 NAA（0.2mg/l）的基础培养基上。再生的芽在添加 NAA 的基础培养基上诱导生根。在体外获得的植株被转移到土壤中，并成功生长到成熟。在再生的 dicoccum 植株中观察到叶绿素变体。
