从蓝龙胆 Gentiana lutea L.的贮藏根原生质体的细胞质和液泡中蔗糖和龙胆二糖的差异区室化

Differential compartmentation of sucrose and gentianose in the cytosol and vacuoles of storage root protoplasts from Gentiana Lutea L.

机构信息

Dept. of General Botany, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland.

出版信息

Plant Cell Rep. 1982 Dec;1(6):274-7. doi: 10.1007/BF00272638.

Abstract

The storage roots of perennial Gentiana lutea L.plants contain several sugars. The predominant carbohydrate reserve is gentianose (β-D-glucopyranosyl-(1 → 6)-α-D-glucopyranosyl-(1 ↔ 2)-β-D-fructofuranoside). Vacuoles were isolated from root protoplasts and purified through a betaine density gradient. The yield was about 75%. Gentianose and gentiobiose were localized to 100% in the vacuoles, fructose and glucose to about 80%, and sucrose to only about 50%. Taking the volumes of the vacuolar and extravacuolar (cytosolic) compartments into account it is inferred that gentianose is located exclusively in the vacuoles, whilst sucrose is much more concentrated in the cytosol where it may play a role as a cryoprotectant. The concentration of fructose and glucose appeared to be similar on both sides of the tonoplast.

摘要

多年生龙胆属植物的块根含有几种糖。主要的碳水化合物储备是龙胆二糖（β-D-吡喃葡萄糖基-(1 → 6)-α-D-吡喃葡萄糖基-(1 ↔ 2)-β-D-呋喃果糖苷）。从根原生质体中分离出液泡，并通过甜菜碱密度梯度进行纯化。产量约为 75%。龙胆二糖和龙胆三糖 100%定位在液泡中，果糖和葡萄糖约 80%，蔗糖仅约 50%。考虑到液泡和细胞外区室（细胞质）的体积，可以推断龙胆二糖仅存在于液泡中，而蔗糖在细胞质中浓度更高，可能在其中作为抗冻保护剂发挥作用。液泡膜两侧的果糖和葡萄糖浓度似乎相似。