将大肠杆菌细胞和原生质球导入长春花原生质体。
Introduction of Escherichia coli cells and spheroplasts into Vinca protoplasts.
机构信息
Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya.
出版信息
Plant Cell Rep. 1983 Feb;2(1):30-2. doi: 10.1007/BF00269230.
PMID:24257851
Abstract
In the presence of 10% polyvinyl alcohol (PVA), Escherichia coli cells or spheroplasts can be easily introduced into Vinca protoplasts by endocytosis. Uptake proceeded quite rapidly; bacterial cells or spheroplasts were found within the cytoplasm of Vinca protoplasts after 10 min of incubation with PVA.
摘要
在 10%聚乙烯醇（PVA）的存在下，通过胞吞作用，大肠杆菌细胞或原生质体可以很容易地被导入长春花原生质体中。摄取过程进行得相当迅速；在用 PVA 孵育 10 分钟后，细菌细胞或原生质体被发现位于长春花原生质体的细胞质内。
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