提高原生质体平板效率和克隆选择的新程序。
A new procedure for increasing efficiency of protoplast plating and clone selection.
机构信息
International Plant Research Institute, 94070, San Carlos, CA, USA.
出版信息
Plant Cell Rep. 1983 Aug;2(4):165-8. doi: 10.1007/BF00270093.
PMID:24258041
Abstract
Sea Prep(TM) agarose (FMC corp.) was used as a gelling agent for media in the culture of potato and tomato protoplasts. The concentration and conditions appropriate for gelling and liquifaction were determined. The new method provided increased calli transfer efficiency of up to 50-fold. Shoot formation from the protoplast-derived cells occurred normally and without reduction in frequency.
摘要
海备（TM）琼脂糖（FMC 公司）被用作培养马铃薯和番茄原生质体的培养基的凝胶剂。确定了凝胶化和液化的合适浓度和条件。新方法提供了高达 50 倍的愈伤组织转移效率的提高。由原生质体衍生的细胞的芽形成正常发生，频率没有降低。
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