从小麦中分离和鉴定在酿酒酵母中起复制起点作用的 DNA 序列。
Isolation and characterization of DNA sequences from Triticum aestivum which function as origins of replication in Saccharomyces cerevisiae.
机构信息
Chaire de Physiologie Animale et de Zootechnie, Faculté des Sciences Agronomiques de l'Etat, B-5800, Gembloux, Belgium.
出版信息
Plant Cell Rep. 1983 Aug;2(4):175-8. doi: 10.1007/BF00270096.
PMID:24258044
Abstract
Recombinant YIp5 plasmids with the DNA from Triticum aestivum are capable of autonomous replication in Saccharomyces cerevisiae. The URA transformants are unstable without selection pressure, and transformation of yeast cells with these plasmids occurs at high frequency. The cloned sequences were characterized and analyzed to state their belonging to Triticum tribe.
摘要
含有小麦 DNA 的重组 YIp5 质粒能够在酿酒酵母中自主复制。URA 转化体在没有选择压力的情况下不稳定，并且这些质粒转化酵母细胞的频率很高。对克隆序列进行了特征分析，以确定其属于小麦族。
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