从万氏甲烷球菌中克隆能够在酵母中自主复制的DNA序列。
Cloning of DNA sequences from Methanococcus vannielii capable of autonomous replication in yeast.
作者信息
Meile L, Leisinger T, Reeve J N
出版信息
Arch Microbiol. 1985 Dec;143(3):253-5. doi: 10.1007/BF00411245.
Total DNA of the archaebacterium Methanococcus vannielii was digested with BamHI or BamHI/HindIII, cloned with plasmid Yip5 and analyzed for sequences capable of autonomous replication (ARSs) in the eukaryote Saccharomyces cerevisiae. Two recombinant plasmids were isolated which contained 3.3 kb and 8 kb fragments of methanogen derived DNA with ARS activity. They exhibited low transformation efficiencies for yeast and promoted slow growth of yeast transformants.
嗜甲烷球菌（Methanococcus vannielii）的总DNA用BamHI或BamHI/HindIII进行消化，与质粒Yip5进行克隆，并分析其在真核生物酿酒酵母（Saccharomyces cerevisiae）中能够自主复制的序列（ARSs）。分离出两个重组质粒，它们含有具有ARS活性的3.3 kb和8 kb产甲烷菌衍生DNA片段。它们对酵母的转化效率较低，并导致酵母转化子生长缓慢。
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