International Plant Research Institute, 94070, San Carlos, CA, USA.
Plant Cell Rep. 1983 Aug;2(4):213-5. doi: 10.1007/BF00270107.
Protoplasts were isolated from cell cultures of G. soja and G. tabacina, respectively. The isolation procedure employed Percoll for the separation and concentration of protoplasts. The cultured protoplasts formed cells which developed into embryo-like structures. Protoplasts also were isolated from leaf tissue of soybean cv. Williams 82. Upon culture, the protoplasts regenerated cell walls and divided to form cell cultures.
原生质体分别从大豆和烟草的细胞培养物中分离出来。采用聚蔗糖（Percoll）进行原生质体的分离和浓缩。培养的原生质体形成细胞，这些细胞进一步发育成类胚结构。也从大豆品种 Williams 82 的叶片组织中分离原生质体。培养后，原生质体再生细胞壁并分裂形成细胞培养物。
