Department of Ecology, University of Lund, S-225 62, Lund, Sweden.
J Chem Ecol. 1991 Nov;17(11):2113-25. doi: 10.1007/BF00987995.
The olfactorial response of the fungivorous soil collembolanOnychiurus armatus was examined in a bioassay covering volatile compounds identified in the odor blends of two of its preferred fungal speciesMonierella isabellina andVerticillium bulbillosum. The odor of the fungi was trapped using activated carbon filters, extracted with diethyl ether, and subjected to GC-MS analysis. About 50% of the compounds resolved by GC were identified by a combination of electron impact and chemical ionization mass spectrometry. In a Y-shaped olfactometer the collembolans were attracted to a variety of common odors, such as CO2 and 2-methyl-1-propanol, and a species-specific odor, such as 1-heptene, and arrested by, for example, decanal and 2-octene. The response was not improved by pairwise combinations of common and specific odors. An amount of 0.5 ng of ethyl acetate or 3 pg of 1-pentanol was sufficient to attract the collembolans. The specific compounds ofV. bulbillosum, 1-heptene and 1-octen-3-ol, may be key stimuli explaining whyO. armatus prefersV. bulbillosum.
本文采用生物测定法，研究了食真菌土壤跳虫 Onychiurus armatus 对两种其偏好真菌（Monierella isabellina 和 Verticillium bulbillosum）气味混合物中鉴定出的挥发性化合物的嗅觉反应。真菌的气味通过活性炭过滤器捕获，用二乙醚提取，并进行 GC-MS 分析。通过电子轰击和化学电离质谱的组合，大约 50%的 GC 分离出的化合物被鉴定。在 Y 型嗅觉仪中，跳虫被各种常见气味（如 CO2 和 2-甲基-1-丙醇）和一种特定物种的气味（如 1-庚烯）吸引，并被例如癸醛和 2-辛烯阻止。常见和特定气味的成对组合并不能提高反应。0.5ng 的乙酸乙酯或 3pg 的 1-戊醇足以吸引跳虫。V. bulbillosum 的特定化合物 1-庚烯和 1-辛烯-3-醇可能是解释为什么 O. armatus 偏好 V. bulbillosum 的关键刺激物。
