同工酶变异的遗传基础碱性磷酸酶和葡萄糖磷酸异构酶在茄属植物中。

Genetic bases of isozyme variation for alkaline phosphatase and glucosephosphate isomerase in Solanum.

机构信息

Department of Horticulture, The Pennsylvania State University, 16802, University Park, PA, USA.

出版信息

Theor Appl Genet. 1984 Apr;67(6):505-13. doi: 10.1007/BF00264894.

Abstract

Genetic bases of isozyme phenotypes of alkaline phosphatase (AKP) and glucosephosphate isomerase (GpI) from tuber extracts of potato species of the genus Solanum were investigated by starch gel electrophoresis. Data were obtained from reciprocal F1 matings of S. tuberosum X ssp. andigena (Juz. & Buk.) Hawkes and ssp. tuberosum X (S. phureja X S. chacoense) and BC1 matings where ssp. tuberosum was the recurrent parent. AKP and GPI are dimeric enzymes and the variation observed for each was found to be coded by single tetrasomic loci (Akp and Gpi) with three (A, A', A″) and five (G, G', G″, G‴, G[Symbol: see text]) alleles, respectively. Although the G‴ and G[Symbol: see text] encoded homodimers have similar electrophoretic mobilities, the specific enzymatic activity of the G[Symbol: see text] encoded homodimer is approximately 25% that of the G‴. The predictable genetic bases for these two enzymatic polymorphisms make them suitable for use as genetic markers in the potato. Chromosome mapping of the loci which encode these enzymes is now possible.

摘要

利用淀粉凝胶电泳技术研究了来自茄属马铃薯种块茎提取物中碱性磷酸酶（AKP）和葡萄糖磷酸异构酶（GpI）同工酶表型的遗传基础。数据来自 S. tuberosum X ssp. andigena（Juz. & Buk.）Hawkes 和 ssp. tuberosum X（S. phureja X S. chacoense）的正反交 F1 杂种以及以 ssp. tuberosum 为轮回亲本的 BC1 杂种。AKP 和 GPI 是二聚酶，观察到的每种同工酶的变化被发现由单个四体基因座（Akp 和 Gpi）编码，分别具有三个（A、A'、A″）和五个（G、G'、G″、G‴、G[Symbol: see text]）等位基因。虽然编码 G‴和 G[Symbol: see text]的同源二聚体具有相似的电泳迁移率，但 G[Symbol: see text]编码的同源二聚体的特定酶活性约为 G‴的 25%。这两种酶多态性的可预测遗传基础使它们成为马铃薯中遗传标记的合适选择。现在可以对编码这些酶的基因座进行染色体作图。