体外筛选对 TMV 具有有限抗性的野生烟草变异体。

In vitro selection of Nicotiana sylvestris variants with limited resistance to TMV.

机构信息

Department of Botany and Plant Pathology, Michigan State University, 48824, East Lansing, Michigan, USA.

出版信息

Plant Cell Rep. 1982 Apr;1(3):94-7. doi: 10.1007/BF00272361.

Abstract

Haploid tobacco (Nicotiana sylvestris) plants were inoculated with a yellow strain of tobacco mosaic virus (TMV-Flavum) and then exposed to 500 rads of acute gamma radiation. Leaf strips cultured on callus-inducing medium yielded two types of colonies: 1) yellow, virus-infected and 2) green, apparently healthy. Of the 3210 calli scored, approximately 5% were virus-free, and after regeneration, 0.2% were resistant at the plant stage. Later, adult plants, both TMV-resistant and TMV-susceptible, produced self-fertile, diploid flowers. Both seedling progeny and rooted cuttings from resistant stock plants showed resistance to TMV infection. This resistance was characterized by restricted virus multiplication and movement within the infected plant resulting in a 3-8 week delay in symptom expression.

摘要

单倍体烟草（Nicotiana sylvestris）植株被黄花烟草花叶病毒（TMV-Flavum）接种，然后暴露于 500 拉德的急性γ辐射下。在愈伤组织诱导培养基上培养的叶条产生了两种类型的菌落：1）黄色，病毒感染；2）绿色，明显健康。在 3210 个愈伤组织中，约有 5%是无病毒的，再生后，在植物阶段有 0.2%是抗性的。后来，抗 TMV 和易感 TMV 的成年植株都产生了自交可育的二倍体花。抗株系的幼苗后代和生根插条都表现出对 TMV 感染的抗性。这种抗性的特点是病毒在感染植物内的增殖和移动受到限制，导致症状表现延迟 3-8 周。