LaMondia J A
The Connecticut Agricultural Experiment Station, Valley Laboratory, Windsor 06095.
Plant Dis. 2008 Jan;92(1):37-41. doi: 10.1094/PDIS-92-1-0037.
Near-isogenic lines of cigar wrapper tobacco resistant or susceptible to Tobacco mosaic virus (TMV) were used to evaluate the association of TMV infection with green spot symptoms in cured leaves. TMV infection, as determined by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), was detected on susceptible but not resistant plants in field experiments. Green spot severity on cured leaves was greater for susceptible than resistant plants, even when symptoms of TMV were not evident in the field. Some green spots were present on resistant leaves despite the fact that the virus was not detected by ELISA. Resistant and susceptible plants had similar responses to virus infection and similar ELISA detection of TMV when plants were held at continuous temperatures over 28°C in growth chambers. Plant resistance was not compromised in the field in cloth-covered shade tents even when 33.5 of the 96 h immediately following inoculation were above 28°C. Green spot of cured leaves was strongly associated with TMV infection in susceptible plants, even when plants were infected after leaf expansion and mosaic symptoms were not present. Green spot also occurred to a lesser extent and for a limited time in inoculated resistant plants. The development of green spot symptoms on cured leaves may be the result of either systemic infection of TMV-susceptible plants or associated with the systemic resistance response to TMV inoculation of resistant plants.
利用对烟草花叶病毒（TMV）具有抗性或敏感性的雪茄外包叶烟草近等基因系，评估TMV感染与调制后烟叶绿斑症状之间的关联。在田间试验中，通过双抗体夹心酶联免疫吸附测定（ELISA）确定，在感病植株而非抗病植株上检测到了TMV感染。即使在田间TMV症状不明显时，感病植株调制后烟叶上的绿斑严重程度也高于抗病植株。尽管ELISA未检测到病毒，但抗病烟叶上仍出现了一些绿斑。当植株在生长室中保持在28°C以上的持续温度时，抗病和感病植株对病毒感染的反应相似，对TMV的ELISA检测结果也相似。即使在接种后的96小时中有33.5小时温度高于28°C，在田间用布覆盖的遮荫帐篷中，植株抗性也未受到损害。在感病植株中，即使植株在叶片展开后被感染且不存在花叶症状，调制后烟叶的绿斑也与TMV感染密切相关。在接种的抗病植株中，绿斑也会在较小程度上出现且持续时间有限。调制后烟叶上绿斑症状的出现可能是TMV感病植株系统感染的结果，或者与抗病植株对TMV接种的系统抗性反应有关。
