Lopez J A, Guillen M, Sanchez-Fauquier A, Melero J A
J Virol Methods. 1986 Jun;13(3):255-64. doi: 10.1016/0166-0934(86)90019-4.
A simple antigen-binding assay is described for determining the specificity of monoclonal antibodies against A/Victoria/3/75 influenza virus. Monoclonal antibodies were bound to polyvinylchloride microtitre wells via protein A and anti-immunoglobulin serum. Radiolabelled cell extracts were then added and allowed to adsorb to the antigen. The bound material was eluted and analyzed by polyacrylamide gel electrophoresis and autoradiography. The method can also be used to study competitive binding of antibodies to a given antigen. In this case, one antibody was adsorbed to the wells as above and the competitor antibody was added in high excess with the radioactive antigen. The results obtained with this method are comparable to those obtained by competitive RIA or ELISA.
描述了一种简单的抗原结合试验，用于确定抗A/维多利亚/3/75流感病毒单克隆抗体的特异性。单克隆抗体通过蛋白A和抗免疫球蛋白血清结合到聚氯乙烯微量滴定孔上。然后加入放射性标记的细胞提取物，使其吸附到抗原上。洗脱结合的物质，并通过聚丙烯酰胺凝胶电泳和放射自显影进行分析。该方法还可用于研究抗体与给定抗原的竞争性结合。在这种情况下，一种抗体按上述方法吸附到孔中，竞争抗体与放射性抗原一起以高过量加入。用该方法获得的结果与通过竞争性放射免疫分析（RIA）或酶联免疫吸附测定（ELISA）获得的结果相当。
