Department of Botany, University of Durham, DH1 3LE, Durham, U.K..
Planta. 1979 Jan;146(1):63-9. doi: 10.1007/BF00381256.
The changes in endoplasmic reticulum (ER) morphology during seed development have been followed using a thick section electron microscope technique. The tissues were stained with a zinc iodineosmium tetroxide complex which preferentially accumulated in the lumen between double membranes. Sections up to 2 μm in thickness were examined in a high voltage electron microscope (HVEM) with tilt facility to produce stereo pairs. The micrographs from HVEM showed an increase in the extent of interconnecting tubular and cisternal ER during the protein deposition phase of seed maturation with subsequent degeneration of the cisternae to a reticular form during the final seed maturation phase. No evidence of cisternal ER vesicles was found, instead our work suggests that such structures are artefacts of thin sectioning with the so-called vesicles representing the interconnection of cisternal and tubular ER. The results are discussed with reference to the transport of storage protein from its site of synthesis, the rough cisternal ER, to that of accumulation, the vacuolar protein bodies.
使用厚切片电子显微镜技术研究了种子发育过程中内质网（ER）形态的变化。组织用锌碘锇四氧化物复合物染色，该复合物优先积累在双层膜之间的腔中。使用带有倾斜装置的高电压电子显微镜（HVEM）检查厚度达 2 μm 的切片，以产生立体对。HVEM 的显微照片显示，在种子成熟的蛋白质沉积阶段，管状和腔内质网的连接程度增加，随后在种子成熟的最后阶段，腔被退化形成网状。没有发现腔内质网小泡的证据，相反，我们的工作表明，这种结构是薄切片的人为产物，所谓的小泡代表腔内质网和管状内质网的连接。结果与从粗面内质网的储存蛋白合成部位到积累部位液泡蛋白体的储存蛋白运输有关。
