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An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapain.

作者信息

Sluyterman L A, Wijdenes J

出版信息

Biochim Biophys Acta. 1970 Mar 31;200(3):593-5. doi: 10.1016/0005-2795(70)90122-4.

DOI:10.1016/0005-2795(70)90122-4
PMID:5436650
Abstract
摘要

相似文献

1
An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapain.一种用于分离巯基木瓜蛋白酶和非巯基木瓜蛋白酶的琼脂糖汞柱。
Biochim Biophys Acta. 1970 Mar 31;200(3):593-5. doi: 10.1016/0005-2795(70)90122-4.
2
The purification of papain by affinity chromatography.通过亲和色谱法纯化木瓜蛋白酶。
Eur J Biochem. 1970 Jul;15(1):97-102. doi: 10.1111/j.1432-1033.1970.tb00981.x.
3
Determination of protein thiol groups by atomic absorption spectrometry.用原子吸收光谱法测定蛋白质硫醇基团
Anal Biochem. 1969 Oct 15;32(1):106-17. doi: 10.1016/0003-2697(69)90109-2.
4
Reaction of protein disulfide groups with Ellman's reagent: a case study of the number of sulfhydryl and disulfide groups in Aspergillus oryzae -amylase, papain, and lysozyme.蛋白质二硫键基团与埃尔曼试剂的反应:以米曲霉α淀粉酶、木瓜蛋白酶和溶菌酶中巯基和二硫键基团数量为例的研究
Arch Biochem Biophys. 1971 Nov;147(1):262-9. doi: 10.1016/0003-9861(71)90334-1.
5
Benzoylamidoacetonitrile as an inhibitor of papain.苯甲酰氨基乙腈作为木瓜蛋白酶的抑制剂。
Biochim Biophys Acta. 1973 Mar 15;302(1):95-101. doi: 10.1016/0005-2744(73)90012-0.
6
Chemical modification of papain. I. Reaction with the chloromethyl ketones of phenylalanine and lysine and with phenylmethyl-sulfonyl fluoride.木瓜蛋白酶的化学修饰。I. 与苯丙氨酸和赖氨酸的氯甲基酮以及苯甲基磺酰氟的反应。
Arch Biochem Biophys. 1968 Mar 20;124(1):70-8. doi: 10.1016/0003-9861(68)90304-4.
7
Organomercurial agarose.
Methods Enzymol. 1974;34:544-7. doi: 10.1016/s0076-6879(74)34070-0.
8
Completion of the amino acid sequence of papain.木瓜蛋白酶氨基酸序列的测定完成。
Biochem J. 1969 Sep;114(2):279-88. doi: 10.1042/bj1140279.
9
Chymopapain. IV. The chromatographic fractionation of partially purified chymopapain and the characterization of crystalline chymopapain B.
Biochim Biophys Acta. 1967 Jul 11;139(2):405-17. doi: 10.1016/0005-2744(67)90044-7.
10
Modified preparation of thiolsubtilisins and their purification on Agarose-mercurial column.巯基枯草杆菌蛋白酶的改良制备及其在琼脂糖汞柱上的纯化
Acta Biochim Biophys Acad Sci Hung. 1976;11(2-3):81-6.

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1
Origin and development of protein bodies in cotyledons of Vicia faba : Proposal for an uniform mechanism.蚕豆子叶中蛋白体的起源和发育:统一机制的提出。
Planta. 1983 Apr;157(5):401-10. doi: 10.1007/BF00397197.
2
Protein bodies from the cotyledons of Cytisus scoparius L. (Link). Ultrastructure, isolation, and subunit composition of albumin, legumin and vicilin.金雀花豆科菜豆属(Link)子叶中的蛋白体。白蛋白、豆球蛋白和伴大豆球蛋白的超微结构、分离和亚基组成。
Planta. 1985 Sep;166(1):39-45. doi: 10.1007/BF00397383.
3
Peptidyl cyclopropenones: reversible inhibitors, irreversible inhibitors, or substrates of cysteine proteases?
肽环丙烯酮:半胱氨酸蛋白酶的可逆抑制剂、不可逆抑制剂还是底物?
Protein Sci. 2013 Jun;22(6):788-99. doi: 10.1002/pro.2260. Epub 2013 Apr 29.
4
Inactivation of cysteine proteases by peptidyl epoxides: characterization of the alkylation sites on the enzyme and the inactivator.肽基环氧化物对半胱氨酸蛋白酶的失活作用:酶和失活剂上烷基化位点的表征
Biochem J. 2000 Feb 15;346 Pt 1(Pt 1):71-6.
5
Pharmacokinetics of oral tiopronin.口服硫普罗宁的药代动力学
Eur J Clin Pharmacol. 1993;45(1):79-84. doi: 10.1007/BF00315354.
6
E64 [trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane] analogues as inhibitors of cysteine proteinases: investigation of S2 subsite interactions.作为半胱氨酸蛋白酶抑制剂的E64 [反式环氧琥珀酰-L-亮氨酰胺基-(4-胍基)丁烷]类似物:S2亚位点相互作用的研究
Biochem J. 1994 Apr 15;299 ( Pt 2)(Pt 2):389-92. doi: 10.1042/bj2990389.
7
Local pH-dependent conformational changes leading to proteolytic susceptibility of cystatin C.导致胱抑素C产生蛋白水解敏感性的局部pH依赖性构象变化。
Biochem J. 1994 Sep 1;302 ( Pt 2)(Pt 2):411-6. doi: 10.1042/bj3020411.
8
Purification and partial characterization of a thiol proteinase from the thermophilic fungus Humicola lanuginosa.嗜热真菌羊毛状腐质霉硫醇蛋白酶的纯化及部分特性分析
Biochem J. 1982 Jul 1;205(1):147-52. doi: 10.1042/bj2050147.
9
Subsite differences between the active centres of papaya peptidase A and papain as revealed by affinity chromatography. Purification of papaya peptidase A by ionic-strength-dependent affinity adsorption on an immobilized peptide inhibitor of papain.通过亲和色谱揭示木瓜蛋白酶A和木瓜蛋白酶活性中心的亚位点差异。利用木瓜蛋白酶固定化肽抑制剂上离子强度依赖性亲和吸附法纯化木瓜蛋白酶A。
Biochem J. 1984 May 1;219(3):727-33. doi: 10.1042/bj2190727.
10
The in vivo and in vitro initiation site for transcription of the rRNA operon of Saccharomyces carlsbergensis.卡尔斯伯酵母rRNA操纵子转录的体内和体外起始位点。
Nucleic Acids Res. 1984 Feb 10;12(3):1377-90. doi: 10.1093/nar/12.3.1377.