Ikegaki N, Bukovsky J, Kennett R H
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5929-33. doi: 10.1073/pnas.83.16.5929.
Increased N-myc (now designated NMYC in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. As yet, the N-myc gene product has not been identified. To detect and characterize the N-myc gene product, we have developed monoclonal antibodies against the putative N-myc gene product made in Escherichia coli as a fusion protein. The antibodies that recognize the N-myc-specific regions were selected on the basis of their reactivities to different portions of the fusion protein. These monoclonal antibodies detect a pair of closely migrating polypeptides of 60 and 63 kDa in nuclear fractions of human neuroblastoma cells. The relative levels of the polypeptides are roughly proportional to the level of N-myc transcripts present in a panel of neuroblastoma lines. These two polypeptides have a half-life of approximately equal to 35 min, and they are indistinguishable from each other by their epitopic profiles.
在某些类型的肿瘤中，已在转录水平检测到N-myc（现根据人类基因命名法命名为NMYC）基因表达增加。到目前为止，尚未鉴定出N-myc基因产物。为了检测和表征N-myc基因产物，我们制备了针对在大肠杆菌中作为融合蛋白产生的假定N-myc基因产物的单克隆抗体。基于它们对融合蛋白不同部分的反应性，选择了识别N-myc特异性区域的抗体。这些单克隆抗体在人神经母细胞瘤细胞的核部分中检测到一对迁移紧密的60 kDa和63 kDa多肽。这些多肽的相对水平大致与一组神经母细胞瘤细胞系中存在的N-myc转录本水平成正比。这两种多肽的半衰期约为35分钟，并且根据它们的表位谱无法区分彼此。
