Chiba M
Department of Epidemiology and Environmental Health, Juntendo University School of Medicine.
Nihon Rinsho. 1996 Jan;54(1):179-85.
Research on trace elements has been paid attention recently. In order to determine concentrations of various kinds of trace elements in biological materials, atomic absorption spectrophotometer is widely used. This instrument allows single element analysis at ppb levels. Microwave induced plasma-mass spectrophotometer (MIP-MS) or inductively coupled plasma-mass spectrophotometer (ICP-MS) permits multielement analysis at sub-ppb or ppt levels. These analytical instruments accept liquid samples only. Biological materials such as organs, tissue and blood must be digested in prior to apply into the instrument. Digesting methods, simplified wet ashing and microwave ashing are introduced.
近年来，微量元素的研究受到了关注。为了测定生物材料中各种微量元素的浓度，原子吸收分光光度计被广泛使用。该仪器可对十亿分之一水平的单一元素进行分析。微波诱导等离子体质谱仪（MIP-MS）或电感耦合等离子体质谱仪（ICP-MS）能够对亚十亿分之一或万亿分之一水平的多种元素进行分析。这些分析仪器仅接受液体样品。诸如器官、组织和血液等生物材料在应用于仪器之前必须进行消化处理。本文介绍了简化湿灰化和微波灰化等消化方法。
