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尿5-羟吲哚-3-乙酸的测定:两种方法的比较

Assay of urinary 5-hydroxyindole-3-acetic acid: two methodologies compared.

作者信息

Baars J D, van Haard P M, Lombarts A J

出版信息

Clin Chim Acta. 1986 Jul 30;158(2):173-8. doi: 10.1016/0009-8981(86)90233-0.

DOI:10.1016/0009-8981(86)90233-0
PMID:2427263
Abstract

Udenfriend's (Sjoerdsma A, Weissbach H, Udenfriend S. J Am Med Assoc 1955; 159: 397) classical screening method for urinary 5-hydroxyindole-3-acetic acid (5-HIAA) is known to be subject to error due to food and drug interferences. Goldenberg (Goldenberg H. Clin Chem 1973; 19: 38-44) introduced a more specific and sensitive method, which has recently been modified by Zouheir Habbal (Zouheir Habbal M. Clin Chim Acta 1983; 130: 251-256). The latter method is shown to correlate fairly well with a high-pressure liquid chromatography (HPLC) method in the 0-100 mumol/24 h range (reference values up to 34 mumol/24 h). It is suggested that elevated values in Udenfriend's screening method can be reliably checked by applying either method.

摘要

已知乌登弗里德(Sjoerdsma A、Weissbach H、Udenfriend S。《美国医学会杂志》1955年;159:397)经典的尿5-羟吲哚-3-乙酸(5-HIAA)筛查方法会因食物和药物干扰而出现误差。戈尔登伯格(Goldenberg H。《临床化学》1973年;19:38 - 44)引入了一种更特异、更灵敏的方法,该方法最近由祖海尔·哈巴尔(Zouheir Habbal M。《临床化学学报》1983年;130:251 - 256)进行了改进。结果表明,后一种方法在0 - 100 μmol/24小时范围内(参考值高达34 μmol/24小时)与高压液相色谱(HPLC)法有较好的相关性。建议通过应用这两种方法中的任何一种,都可以可靠地检查乌登弗里德筛查方法中升高的值。

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