Tracy R P, Wold L E, Jones J D, Burritt M F
Clin Chem. 1981 Jan;27(1):160-2.
The popular colorimetric assay for urinary 5-hydroxyindole-3-acetic acid based on the reaction with 1-nitroso-2-naphthol has been criticized for lack of specificity. Goldenberg (Clin. Chem. 19: 38-44, 1973) proposed a modification of this method, in which 2-mercaptoethanol is used to discharge unwanted chromophores. We compared results by both the unmodified and modified colorimetric methods with those by a "high-performance" liquid-chromatographic method, with fluorometric detection. Agreement among all three assays was excellent in the range of 50 to 600 mg/24 h, but there were considerable differences in the range 0 to 30 mg/24 h between results by the unmodified colorimetric assay and the other two. In this range, results by the modified assay (y) correlated well with those by the chromatographic method (x), although the values were slightly lower (y = 0.85x + 0.11, r = 0.9303). Using the modified colorimetric assay to analyze 40 normal specimens, we defined a normal range of 0 to 6 mg/24 h). We recommend the modified colorimetric assay for use in the high-volume laboratory and either this assay or the liquid-chromatographic method for the smaller laboratory.
基于与1-亚硝基-2-萘酚反应的尿5-羟吲哚-3-乙酸比色测定法因缺乏特异性而受到批评。戈德堡（《临床化学》19: 38 - 44, 1973）提出了该方法的一种改进，其中使用2-巯基乙醇去除不需要的发色团。我们将未改进和改进的比色法结果与采用荧光检测的“高效”液相色谱法结果进行了比较。在50至600 mg/24 h范围内，三种测定方法的结果一致性良好，但在0至30 mg/24 h范围内，未改进的比色测定法与其他两种方法的结果存在显著差异。在此范围内，改进测定法的结果（y）与色谱法的结果（x）相关性良好，尽管数值略低（y = 0.85x + 0.11，r = 0.9303）。使用改进的比色测定法分析40份正常标本，我们确定正常范围为0至6 mg/24 h。我们建议在大容量实验室使用改进的比色测定法，在较小的实验室使用该测定法或液相色谱法。
