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小麦胚凝集素。多种形式存在遗传基础的证据。

Wheat germ agglutinin. Evidence for a genetic basis of multiple forms.

作者信息

Rice R H

出版信息

Biochim Biophys Acta. 1976 Aug 24;444(1):175-80. doi: 10.1016/0304-4165(76)90234-8.

DOI:10.1016/0304-4165(76)90234-8
PMID:953023
Abstract

Germ from hexaploid wheat (Triticum aestivum L.) contained three forms of agglutinin separable by ion-exchange column chromatography at pH 3.8, while germ from tetraploid wheat (Triticum turgidum L. (durum group)) contained only two such forms. The different number of forms, not due to protein modification occurring during the purification process, was demonstrable in commercial germ and in bran fractions containing germ from single wheat varieties. This evidence for a genetic basis of lectin multiple forms in wheat indicates the advisability of using genetically identified plant varieties in lectin research.

摘要

六倍体小麦(普通小麦)的胚中含有三种可通过离子交换柱色谱在pH 3.8条件下分离的凝集素形式,而四倍体小麦(硬粒小麦(硬粒组))的胚中仅含有两种这样的形式。在商业胚以及含有单一小麦品种胚的麸皮组分中都能证明,这种形式数量的差异并非由于纯化过程中发生的蛋白质修饰所致。小麦凝集素多种形式的这种遗传基础证据表明,在凝集素研究中使用经过基因鉴定的植物品种是明智的。

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Wheat germ agglutinin. Evidence for a genetic basis of multiple forms.小麦胚凝集素。多种形式存在遗传基础的证据。
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引用本文的文献

1
A genetic basis for the origin of six different isolectins in hexaploid wheat.六倍体小麦中六种不同同工凝集素起源的遗传基础。
Planta. 1982 Jun;154(6):562-7. doi: 10.1007/BF00403001.
2
Wheat germ agglutinin accumulation in coleoptiles of different genotypes of wheat. Localization by monoclonal antibodies.不同基因型小麦胚芽鞘中麦胚凝集素的积累。单克隆抗体的定位。
Plant Cell Rep. 1987 Apr;6(2):146-9. doi: 10.1007/BF00276674.
3
Plant as a plenteous reserve of lectin.植物是凝集素的丰富储备。
Plant Signal Behav. 2013;8(12):e26595. doi: 10.4161/psb.26595. Epub 2013 Oct 1.
4
Characterization of Urtica dioica agglutinin isolectins and the encoding gene family.荨麻凝集素同工凝集素及其编码基因家族的特征分析
Plant Mol Biol. 1999 Jan;39(2):335-47. doi: 10.1023/a:1006134932290.
5
Isolation and partial characterization of wheat-germ-agglutinin-like lectins from rye (Secale cereale) and barley (Hordeum vulgare) embryos.从黑麦(Secale cereale)和大麦(Hordeum vulgare)胚中分离并部分鉴定麦胚凝集素样凝集素
Biochem J. 1982 Apr 1;203(1):239-43. doi: 10.1042/bj2030239.
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Sequence variability in three wheat germ agglutinin isolectins: products of multiple genes in polyploid wheat.三种小麦胚芽凝集素同工凝集素中的序列变异性:多倍体小麦中多个基因的产物
J Mol Evol. 1989 Apr;28(4):327-36. doi: 10.1007/BF02103429.
7
Evolution of a family of N-acetylglucosamine binding proteins containing the disulfide-rich domain of wheat germ agglutinin.包含麦胚凝集素富含二硫键结构域的 N-乙酰葡糖胺结合蛋白家族的进化
J Mol Evol. 1991 Sep;33(3):283-94. doi: 10.1007/BF02100680.