Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, IN, USA.
Plant Mol Biol. 1987 Jan;10(1):35-42. doi: 10.1007/BF00014184.
Two full-length (or nearly so) cDNA clones containing information for the protease inhibitors PI IV and C-II from soybean seeds were identified by means of a synthetic probe. DNA sequencing revealed that the two protease inhibitors are synthesized as precursors with a short peptide leader. The coding regions of the two clones show 80% homology, wheraes the 5' non-coding regions are 90% homologous. Homology of 75% is found in the region extending beyond the stop codons.
通过合成探针，鉴定了两个全长（或几乎全长）的 cDNA 克隆，它们包含来自大豆种子的蛋白酶抑制剂 PI IV 和 C-II 的信息。DNA 测序表明，这两种蛋白酶抑制剂作为前体合成，带有一个短肽的先导序列。两个克隆的编码区有 80%的同源性，而 5'非编码区有 90%的同源性。在延伸到终止密码子之外的区域发现有 75%的同源性。
