USDA and the Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA.
Theor Appl Genet. 1985 Aug;70(5):510-9. doi: 10.1007/BF00305984.
DNA clones that encode the group-II subunits of soybean glycinin were identified and compared with clones for group-I subunits. The group-I clones hybridize weakly to those from group-II at low stringency, but fail to hybridize with them at moderate or high stringency. The genes for the group-II subunits are contained in 13 and 9 kb EcoRI fragments of genomic DNA in cultivar CX635-1-1-1. These fragments contain genes for subunits A5A4B3 and A3B4, respectively. The larger size of mature group-II subunits compared with group-I subunits is correlated with a larger sized mRNA. However, the gross arrangement of introns and exons within the group-II coding regions appears to be the same as for the genes which encode group-I subunits. Messenger RNA for both groups of glycinin subunits appear in the seed at the same developmental interval, and their appearance lags slightly behind that of mRNAs for the a/a' subunits of β-conglycinin. These data indicate that the glycinin gene family is more complex than previously thought.
鉴定了编码大豆球蛋白亚基 II 组的 DNA 克隆，并将其与 I 组亚基的克隆进行了比较。在低严谨度下，I 组克隆与 II 组克隆的杂交较弱，但在中或高严谨度下不能与它们杂交。II 组亚基的基因包含在栽培品种 CX635-1-1-1 的 13 和 9kb EcoRI 基因组 DNA 片段中。这些片段分别包含 A5A4B3 和 A3B4 亚基的基因。与 I 组亚基相比，成熟 II 组亚基的大小较大，与其 mRNA 大小相关。然而，编码 II 组亚基的基因内内含子和外显子的总体排列似乎与编码 I 组亚基的基因相同。两种大豆球蛋白亚基的 mRNA 都在种子中出现在相同的发育间隔，并且它们的出现略微落后于β-伴大豆球蛋白的 a/a'亚基的 mRNA。这些数据表明，glycinin 基因家族比以前想象的更为复杂。
