Schlusselhuber Margot, Guldbech Kristen, Sevin Corinne, Leippe Matthias, Petry Sandrine, Grötzinger Joachim, Giguère Steeve, Cauchard Julien
Unit Bacteriology and Parasitology, Dozulé Laboratory for Equine Diseases, Anses, Goustranville, France.
FEMS Microbiol Lett. 2014 Jan;350(2):216-22. doi: 10.1111/1574-6968.12337. Epub 2013 Dec 18.
The equine antimicrobial peptide eCATH1 previously has been shown to have in vitro activity against antibiotic-susceptible reference strains of Rhodococcus equi and common respiratory bacterial pathogens of foals. Interestingly, eCATH1 was also found to be effective in the treatment of R. equi infection induced in mice. The aim of this study was to assess the in vitro activity of eCATH1 against equine isolates of Gram-negative (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae and Pseudomonas spp.) and Gram-positive (R. equi, Staphylococcus aureus) bacteria resistant to multiple classes of conventional antibiotics. A modified microdilution method was used to evaluate the minimum inhibitory concentrations (MICs) of the antimicrobial peptide. The study revealed that eCATH1 was active against all equine isolates of E. coli, S. enterica, K. pneumoniae, Pseudomonas spp. and R. equi tested, with MICs of 0.5-16 μg mL(-1), but was not active against most isolates of S. aureus. In conclusion, the activity of the equine antimicrobial peptide eCATH1 appears to not be hampered by the antibiotic resistance of clinical isolates. Thus, the data suggest that eCATH1 could be useful, not only in the treatment of R. equi infections, but also of infections caused by multidrug-resistant Gram-negative pathogens.
马抗菌肽eCATH1此前已被证明对马红球菌的抗生素敏感参考菌株和马驹常见呼吸道细菌病原体具有体外活性。有趣的是，还发现eCATH1在治疗小鼠诱导的马红球菌感染方面有效。本研究的目的是评估eCATH1对耐多种常规抗生素的革兰氏阴性（大肠杆菌、肠炎沙门氏菌、肺炎克雷伯菌和假单胞菌属）和革兰氏阳性（马红球菌、金黄色葡萄球菌）马源分离株的体外活性。采用改良的微量稀释法评估抗菌肽的最低抑菌浓度（MIC）。研究表明，eCATH1对所有测试的大肠杆菌、肠炎沙门氏菌、肺炎克雷伯菌、假单胞菌属和马红球菌马源分离株均有活性，MIC为0.5 - 16 μg mL(-1)，但对大多数金黄色葡萄球菌分离株无活性。总之，马抗菌肽eCATH1的活性似乎不受临床分离株抗生素耐药性的影响。因此，数据表明eCATH1不仅可用于治疗马红球菌感染，还可用于治疗由多重耐药革兰氏阴性病原体引起的感染。
