LABÉO Frank Duncombe, 14053 Caen, France; Normandie Univ, UNICAEN, U2RM, 14033 Caen, France.
LABÉO Frank Duncombe, 14053 Caen, France.
J Glob Antimicrob Resist. 2019 Dec;19:144-153. doi: 10.1016/j.jgar.2019.03.006. Epub 2019 Mar 15.
This study aimed to analyse antimicrobial susceptibility evolution of equine pathogens isolated from clinical samples from 2006-2016.
A collection of 25 813 bacterial isolates was studied, clustered according to their origins (respiratory tract, cutaneous, genital and other), and analysed for their antimicrobial susceptibility using the disk diffusion method.
The most frequently isolated pathogens were group C Streptococci (27.6%), Escherichia coli (20.0%), Staphylococcus aureus (7.8%), Pseudomonas aeruginosa (4.0%), Enterobacter spp. (3.4%), Klebsiella pneumoniae (2.4%), and Rhodococcus equi (1.8%). Of the isolates, 9512 were from respiratory samples (36.8%), 7689 from genital origin (29.8%), and 4083 from cutaneous samples (15.8%). Over the 11-year period, the frequency of multidrug-resistant (MDR) strains fluctuated between 6.4-20.4% for group C Streptococci and 17-37.7% for Klebsiella pneumoniae. From 2006-2009, 24.5-43.0% of Staphylococcus aureus isolates were MDR; after 2009 the level did not exceeded 27.6%. For Escherichia coli and Enterobacter spp., these levels were mostly >30.0% until 2012, but significantly decreased thereafter (22.5-26.3%).
This study is the first large-scale analysis of equine pathogens, by the number of samples and duration of study. The results showed high levels of MDR strains and the need to support veterinary antimicrobial stewardship to encourage proper use of antibiotics.
本研究旨在分析 2006 年至 2016 年从临床样本中分离的马病原体的抗菌药物敏感性演变。
研究了 25813 株细菌分离株，根据其来源（呼吸道、皮肤、生殖和其他）进行聚类，并使用纸片扩散法分析其抗菌药物敏感性。
最常分离的病原体是 C 群链球菌（27.6%）、大肠杆菌（20.0%）、金黄色葡萄球菌（7.8%）、铜绿假单胞菌（4.0%）、肠杆菌属（3.4%）、肺炎克雷伯菌（2.4%）和马红球菌（1.8%）。在这些分离株中，9512 株来自呼吸道样本（36.8%），7689 株来自生殖系统样本（29.8%），4083 株来自皮肤样本（15.8%）。在 11 年期间，C 群链球菌和肺炎克雷伯菌的耐药菌株频率波动在 6.4%-20.4%和 17%-37.7%之间。2006 年至 2009 年，金黄色葡萄球菌分离株中有 24.5%-43.0%为耐多药菌株；2009 年后，该水平未超过 27.6%。对于大肠杆菌和肠杆菌属，这些水平在 2012 年之前大多超过 30.0%，但此后显著下降（22.5%-26.3%）。
本研究是首次对马病原体进行大规模分析，样本数量和研究时间均创历史新高。结果表明，耐药菌株水平较高，需要支持兽医抗菌药物管理，以鼓励合理使用抗生素。
