National Institute of Plant Genome Research, Aruna Asaf Ali Road, New Delhi 110067, India.
Rice (N Y). 2013 Oct 28;6(1):25. doi: 10.1186/1939-8433-6-25.
Salinity is one of the most common abiotic stresses encountered by plants in the environment and transgenic approaches offer new opportunities to improve tolerance. The mitogen activated protein kinase (MAPK) kinase (MKK) is a key component of MAPK cascade that plays important roles in intra and extra cellular signaling in plants. In the present study, a MKK from rice (Oryza sativa), OsMKK6 was functionally characterized in salt stress by transforming its constitutively active form.
OsMKK6 was made constitutively active by mutating serine and threonine to glutamic acid by site directed mutagenesis, and transformed in indica cultivar rice var. Pusa Basmati-1. The transgenic seedlings growing in 200 mM NaCl solution showed increased root/shoot length and weight, less chlorophyll beaching and higher MAPK activity compared to the wild types.
Present work suggest role of OsMKK6 gene in salt stress signaling in rice.
盐度是植物在环境中遇到的最常见的非生物胁迫之一，转基因方法为提高耐受性提供了新的机会。丝裂原活化蛋白激酶（MAPK）激酶（MKK）是 MAPK 级联反应的关键组成部分，在植物的细胞内和细胞间信号转导中发挥重要作用。在本研究中，通过转化其组成性激活形式，对来自水稻（Oryza sativa）的 MKK，OsMKK6 在盐胁迫下的功能进行了表征。
通过定点突变将丝氨酸和苏氨酸突变为谷氨酸，使 OsMKK6 变为组成性激活形式，并转化到籼稻品种 Pusa Basmati-1 中。与野生型相比，在 200 mM NaCl 溶液中生长的转基因幼苗表现出更长的根/茎长度和重量、较少的叶绿素白化和更高的 MAPK 活性。
本研究表明 OsMKK6 基因在水稻的盐胁迫信号转导中起作用。
