Podleski W K
Am J Med. 1986 Sep;81(3):437-42. doi: 10.1016/0002-9343(86)90295-0.
Spontaneous allergic autocytotoxicity of white blood cells was assessed in six patients with bronchial asthma and 18 normal control volunteers. The observed alterations in non-primed white blood cell membrane were revealed as an increased uptake of trypan blue exclusion dye, an indicator of cell death. The phenomenon of spontaneous allergic autocytotoxicity might be associated with increased permeability of the white blood cell membrane leading to enhanced releasability of chemical mediators of anaphylaxis, which probably bypasses immunoglobulin E mechanisms and T suppressor cell intervention. Of six patients with bronchial asthma, three were sensitive to wheat, two had cows milk sensitivity, and one had corn sensitivity. When white blood cells of these patients were studied in the direct allergic autocytotoxicity assay, augmentation of spontaneous allergic autocytotoxicity by specific food antigens was observed. Two of the patients were professional sports coaches. It is plausible to suggest that increased membrane permeability, presumably indicating increased releasability (i.e., for histamine), may be associated with a low membrane threshold for physical trauma expressed in exercise-induced anaphylaxis syndromes.
对6例支气管哮喘患者和18名正常对照志愿者的白细胞自发过敏性自身细胞毒性进行了评估。在未致敏的白细胞膜中观察到的改变表现为锥虫蓝排斥染料摄取增加，这是细胞死亡的一个指标。自发过敏性自身细胞毒性现象可能与白细胞膜通透性增加有关，导致过敏反应化学介质的释放增加，这可能绕过免疫球蛋白E机制和T抑制细胞干预。在6例支气管哮喘患者中，3例对小麦敏感，2例对牛奶敏感，1例对玉米敏感。当在直接过敏性自身细胞毒性试验中研究这些患者的白细胞时，观察到特定食物抗原增强了自发过敏性自身细胞毒性。其中两名患者是职业体育教练。可以推测，膜通透性增加，可能表明释放性增加（即组胺释放性增加），可能与运动诱发过敏反应综合征中表现出的对物理创伤的低膜阈值有关。
