Podleski W K
Int J Immunopharmacol. 1986;8(4):433-6. doi: 10.1016/0192-0561(86)90128-1.
Spontaneous allergic autocytotoxicity (spACT) of white blood cells (WBC) was assessed in six bronchial asthma patients and eighteen normal control individuals. The observed alterations of non-primed WBC membrane were revealed as an increased uptake of trypan blue exclusion dye, an indicator of death cells. The phenomenon of spACT might be associated with a lack of T suppressor cell intervention, increased refractoriness of WBC membrane leading to its increased permeability and enhanced releasability of chemical mediators of anaphylaxis, which probably bypasses IgE events. In six bronchial asthma patients, three were sensitive toward wheat, two had cow milk sensitivity, and one had corn sensitivity. When WBC of these patients were studied in the direct ACT assay, an additional augmentation of spACT effect by specific food antigens was observed. Surprisingly, Broncho-Vaxom (BX) did not inhibit or enhance spACT. However, BX has antagonistic activity toward direct ACT response in the dose-dependent concentration as previously reported. Our preliminary clinical experience leads us to believe that the spACT assay can serve as a useful clinical discriminator of potential responders versus non-responders to therapy with new agents, when WBC disintegration by autoinduction is involved.
在6名支气管哮喘患者和18名正常对照个体中评估了白细胞（WBC）的自发性过敏自细胞毒性（spACT）。未致敏白细胞膜的观察到的改变表现为锥虫蓝排斥染料摄取增加，这是死亡细胞的一个指标。spACT现象可能与T抑制细胞干预缺乏、白细胞膜难治性增加导致其通透性增加以及过敏反应化学介质的释放性增强有关，这可能绕过了IgE事件。在6名支气管哮喘患者中，3名对小麦敏感，2名对牛奶敏感，1名对玉米敏感。当在直接ACT试验中研究这些患者的白细胞时，观察到特定食物抗原对spACT效应的额外增强。令人惊讶的是，支气管疫苗（BX）既不抑制也不增强spACT。然而，如先前报道，BX对直接ACT反应具有剂量依赖性浓度的拮抗活性。我们的初步临床经验使我们相信，当涉及通过自诱导导致白细胞解体时，spACT试验可作为对新药物治疗潜在反应者与无反应者的有用临床鉴别方法。
