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[Method of staining for lysosomal cationic proteins in histological paraffin sections of biopsies].

作者信息

Pigarevskiĭ V E, Mazing Iu A, Seliverstova V G

出版信息

Arkh Patol. 1986;48(7):82-4.

PMID:2428337
Abstract

A method is suggested for a histochemical and immunofluorescent detection of lysosome cation proteins in the histologic paraffin sections of biopsies. The method is based on the staining of histologic sections obtained from the material fixed in glutaraldehyde buffered with an alcohol solution of a fast green (pH 8.1-8.2) and on the detection myeloperoxidase and lactoferrine by means immune serum by Coons's method. 0.25% solution of azur A (B, C) is used for staining the cell nuclei and viable bacteria.

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