Pigarevskiĭ V E, Mazing Iu A, Seliverstova V G
Arkh Patol. 1986;48(8):77-80.
A method of histochemical identification of cationic proteins, peroxidase, viable and killed bacteria in semithin sections of biopsy samples is suggested. The method is based on dying of araldite-embedded semithin sections with a buffered alcohol solution of fast green (pH 8.15) and on peroxidase identification with O-dianisidine. The method suggested can be used for distinguishing occult forms of infectious gastritis, evaluation of secretory and barrier function of gastric epithelium, selective dying of leucocytes in tissues, revealing of viable and non-viable bacteria in the infective foci.
本文提出了一种在活检样本半薄切片中对阳离子蛋白、过氧化物酶、活细菌和死细菌进行组织化学鉴定的方法。该方法基于用固绿缓冲酒精溶液（pH 8.15）对包埋在环氧树脂中的半薄切片进行染色，以及用过氧二苯胺鉴定过氧化物酶。所提出的方法可用于区分隐匿性感染性胃炎的形式、评估胃上皮的分泌和屏障功能、组织中白细胞的选择性染色、揭示感染灶中的活细菌和死细菌。
