通过部分酸水解和快原子轰击质谱联用进行肽测序。
Peptide sequencing by using a combination of partial acid hydrolysis and fast-atom-bombardment mass spectrometry.
作者信息
De Angelis F, Botta M, Ceccarelli S, Nicoletti R
出版信息
Biochem J. 1986 Jun 1;236(2):609-12. doi: 10.1042/bj2360609.
Abstract
To overcome the limit of the intensity of ions carrying sequence information in structural determinations of peptides by fast-atom-bombardment m.s., we have developed a method that consists in taking spectra of the peptide acid hydrolysates at different hydrolysis times. Peaks correspond to the oligomers arising from the peptide partial hydrolysis. The sequence can then be identified from the structurally overlapping fragments.
摘要
为了克服在通过快原子轰击质谱法进行肽的结构测定时，携带序列信息的离子强度的限制，我们开发了一种方法，该方法包括在不同水解时间获取肽酸水解产物的光谱。峰对应于肽部分水解产生的寡聚物。然后可以从结构上重叠的片段中鉴定序列。
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FAB-mapping of recombinant-DNA protein products.重组DNA蛋白产物的FAB图谱分析。
Biochem Biophys Res Commun. 1983 Nov 30;117(1):299-305. doi: 10.1016/0006-291x(83)91575-9.
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Biochem J. 1982 Jan 1;201(1):105-17. doi: 10.1042/bj2010105.
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Hydrolysis of proteins.蛋白质的水解
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