Jang Jaehee, Kim Youngjun, Hwang Jangsun, Choi Yonghyun, Tanaka Masayoshi, Kang Eunah, Choi Jonghoon
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.
School of Chemical Engineering & Material Science, Chung-Ang University, Seoul 06974, Korea.
Nanomaterials (Basel). 2019 Jul 15;9(7):1016. doi: 10.3390/nano9071016.
Nanodiamonds are emerging as new nanoscale materials because of their chemical stability, excellent crystallinity, and unique optical properties. In this study, the structure of nanodiamonds was engineered to produce carbon nano-onion particles (CNOs) with multiple layers. Following a series of physicochemical characterizations of the CNOs, various evaluations for biological responses were conducted for potential biotechnological applications of the CNOs. The possibility of biological applications was first confirmed by assessment of toxicity to animal cells, evaluation of hemolysis reactions, and evaluation of reactive oxygen species. In addition, human immune cells were evaluated for any possible induction of an immune response by CNOs. Finally, the toxicity of CNOs to present in the human colon was evaluated. CNOs have the chemical and physical properties to be a unique variety of carbon nanomaterials, and their toxicity to animal and human cells is sufficiently low that their biotechnological applications in the future are expected.
由于具有化学稳定性、优异的结晶性和独特的光学性质，纳米金刚石正成为新型的纳米材料。在本研究中，对纳米金刚石的结构进行了改造，以制备具有多层结构的碳纳米洋葱颗粒（CNO）。在对CNO进行了一系列物理化学表征之后，针对CNO在生物技术方面的潜在应用，开展了各种生物学反应评估。通过评估对动物细胞的毒性、溶血反应以及活性氧，首次证实了其生物学应用的可能性。此外，还评估了人类免疫细胞是否会因CNO而引发任何免疫反应。最后，评估了CNO对人结肠中存在的（此处原文似乎不完整）的毒性。CNO具有成为独特种类碳纳米材料的化学和物理性质，并且它们对动物和人类细胞的毒性足够低，因此有望在未来用于生物技术应用。
