Washington University, School of Medicine, Department of Radiology, 4525 Scott Avenue, St. Louis, Missouri 63110.
J Biomed Opt. 2013 Dec;18(12):120501. doi: 10.1117/1.JBO.18.12.120501.
Optical imaging enables real-time visualization of intrinsic and exogenous contrast within biological tissues. Applications in human medicine have demonstrated the power of fluorescence imaging to enhance visualization in dermatology, endoscopic procedures, and open surgery. Although few optical contrast agents are available for human medicine at this time, fluorescence imaging is proving to be a powerful tool in guiding medical procedures. Recently, intraoperative detection of fluorescent molecular probes that target cell-surface receptors has been reported for improvement in oncologic surgery in humans. We have developed a novel system, optical projection of acquired luminescence (OPAL), to further enhance real-time guidance of open oncologic surgery. In this method, collected fluorescence intensity maps are projected onto the imaged surface rather than via wall-mounted display monitor. To demonstrate proof-of-principle for OPAL applications in oncologic surgery, lymphatic transport of indocyanine green was visualized in live mice for intraoperative identification of sentinel lymph nodes. Subsequently, peritoneal tumors in a murine model of breast cancer metastasis were identified using OPAL after systemic administration of a tumor-selective fluorescent molecular probe. These initial results clearly show that OPAL can enhance adoption and ease-of-use of fluorescence imaging in oncologic procedures relative to existing state-of-the-art intraoperative imaging systems.
光学成像是一种实时可视化生物组织内固有和外源性对比的方法。在人类医学中的应用已经证明了荧光成像是增强皮肤科、内窥镜检查和开放性手术可视化的有力手段。尽管目前人类医学中只有少数光学对比剂可用，但荧光成像已被证明是指导医疗程序的有力工具。最近，已经有报道称，荧光分子探针在术中检测到细胞表面受体，这有助于提高人类肿瘤手术的效果。我们开发了一种新系统，即采集荧光的光学投影（OPAL），以进一步增强开放性肿瘤手术的实时指导。在该方法中，收集的荧光强度图被投影到成像表面上，而不是通过壁挂式显示器进行投影。为了证明 OPAL 在肿瘤手术中的应用原理，我们在活体小鼠中可视化了吲哚菁绿的淋巴转运，以便在术中识别前哨淋巴结。随后，在乳腺癌转移的小鼠模型中，在全身给予肿瘤选择性荧光分子探针后，使用 OPAL 识别了腹膜肿瘤。这些初步结果清楚地表明，与现有的最先进的术中成像系统相比，OPAL 可以增强荧光成像在肿瘤手术中的采用和易用性。
