Watson Jeffrey R, Gainer Christian F, Martirosyan Nikolay, Skoch Jesse, Lemole G Michael, Anton Rein, Romanowski Marek
University of Arizona, Department of Biomedical Engineering, 1657 E. Helen Street, Tucson, Arizona 85721, United States.
University of Arizona, Division of Neurosurgery, Department of Surgery, 1501 N. Campbell Avenue, Tucson, Arizona 85721, United States.
J Biomed Opt. 2015 Oct;20(10):106002. doi: 10.1117/1.JBO.20.10.106002.
Intraoperative applications of near-infrared (NIR) fluorescent contrast agents can be aided by instrumentation capable of merging the view of surgical field with that of NIR fluorescence. We demonstrate augmented microscopy, an intraoperative imaging technique in which bright-field (real) and electronically processed NIR fluorescence (synthetic) images are merged within the optical path of a stereomicroscope. Under luminance of 100,000 lx, representing typical illumination of the surgical field, the augmented microscope detects 189 nM concentration of indocyanine green and produces a composite of the real and synthetic images within the eyepiece of the microscope at 20 fps. Augmentation described here can be implemented as an add-on module to visualize NIR contrast agents, laser beams, or various types of electronic data within the surgical microscopes commonly used in neurosurgical, cerebrovascular, otolaryngological, and ophthalmic procedures.
能够将手术视野与近红外（NIR）荧光视野合并的仪器有助于近红外（NIR）荧光造影剂的术中应用。我们展示了增强显微镜，这是一种术中成像技术，其中明场（真实）和电子处理的近红外荧光（合成）图像在立体显微镜的光路内合并。在代表手术视野典型照明的100,000勒克斯亮度下，增强显微镜可检测到189 nM浓度的吲哚菁绿，并以20帧/秒的速度在显微镜目镜内生成真实图像与合成图像的合成图像。此处所述的增强功能可作为一个附加模块来实现，以可视化神经外科、脑血管、耳鼻喉科和眼科手术中常用的手术显微镜内的近红外造影剂、激光束或各种类型的电子数据。
