Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
Mol Imaging. 2010 Oct;9(5):237-55.
The field of biomedical optics has matured rapidly over the last decade and is poised to make a significant impact on patient care. In particular, wide-field (typically > 5 cm), planar, near-infrared (NIR) fluorescence imaging has the potential to revolutionize human surgery by providing real-time image guidance to surgeons for tissue that needs to be resected, such as tumors, and tissue that needs to be avoided, such as blood vessels and nerves. However, to become a clinical reality, optimized imaging systems and NIR fluorescent contrast agents will be needed. In this review, we introduce the principles of NIR fluorescence imaging, analyze existing NIR fluorescence imaging systems, and discuss the key parameters that guide contrast agent development. We also introduce the complexities surrounding clinical translation using our experience with the Fluorescence-Assisted Resection and Exploration (FLARE™) imaging system as an example. Finally, we introduce state-of-the-art optical imaging techniques that might someday improve image-guided surgery even further.
在过去的十年中，生物医学光学领域发展迅速，有望对患者护理产生重大影响。特别是宽场（通常>5cm）、平面、近红外（NIR）荧光成像是一种有潜力的技术，它可以为需要切除的组织（如肿瘤）和需要避开的组织（如血管和神经）提供实时图像引导，从而彻底改变人类手术。然而，为了成为临床现实，需要优化的成像系统和近红外荧光对比剂。在这篇综述中，我们介绍了近红外荧光成像的原理，分析了现有的近红外荧光成像系统，并讨论了指导对比剂开发的关键参数。我们还通过 Fluorescence-Assisted Resection and Exploration（FLARE™）成像系统的经验介绍了临床转化所面临的复杂性。最后，我们介绍了最先进的光学成像技术，这些技术有朝一日可能会进一步提高图像引导手术的效果。
